The extra D
I annotated (marked) for each potential heterozygous web site on the site sequence off parental stresses while the not clear websites by using the suitable IUPAC ambiguity password using a beneficial permissive strategy. I utilized complete (raw) pileup files and you will conservatively thought to be heterozygous web site any webpages that have one minute (non-major) nucleotide in the a frequency greater than 5% no matter what consensus and you may SNP top quality.